Purification and Characterization of Chicken Egg Yolk Antibodies (IgY) Against Mastitis Causing Klebsiella pneumoniae
نویسنده
چکیده
White leghorn hens were immunized intramuscularly with formalin killed Klebsiella pneumoniae cells to generate Klebsiella pneumoniae antibodies (IgY) in egg yolk. Booster injections of increasing concentrations of antigen were given to raise the antibody level in egg yolk. Amount of protein varied from 0.87 7.4mg/ml in egg yolk throughout the immunization period. The antibodies were partially purified from immunized chicken egg yolk by poly ethylene glycol (PEG) and ammonium sulphate precipitation method. Further purification was done by eluting the adsorbed antibodies from DEAE cellulose by linear gradient technique. High titer of more than 1:10000 antibodies were detected by indirect antigen capture ELISA. Inhibition ELISA and growth inhibition assay were also performed to check the effectiveness of IgY. The results indicated that antibodies generated in chicken effectively neutralized mastitis causing Klebsiella pneumoniae and has a potential application in diagnosis and treatment of mastitis causing Klebsiella pneumoniae.
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